Singapore Airlines will mark its retirement of the Boeing 747 after nearly four decades of service with special commemorative flights between Singapore and Hong Kong on April 6.
The route is normally operated with Airbus A330-300s.
Chief executive Goh Choon Phong said that the aircraft's retirement marked "the end of an era" and paid homage to its role in the airline's history.
"Over more than 38 years, the B747 has played an important role in helping Singapore Airlines become the global airline that it is today," he said.
Flying time will be extended by an hour and first class passengers will have the opportunity to tour training facilities and the B747 flight simulator in Singapore.
The flights, now open for sale, will host 300 commercial passengers, along with employees that have worked on the aircraft over the years, disadvantaged children and media.